Informatics Publishing Limited

C. Santhosh Kumar ¹, Chiranjib Bhattacharjee ², Subal Debnath ², G. Ganesh Kumar ², Atul N. Chandu ³

Affiliations
1 Srikrupa Institute of Pharmaceutical Sciences, Vil. Velkatta, Kondapak (mdl), Dist. Medak, Siddipet, Andhra Pradesh – 502 277, IN
2 Srikrupa Institute of Pharmaceutical Sciences, Vil. Velkatta, Kondapak (MDL), Dist. Medak, Siddipet, Andhra Pradesh – 502 277, IN
3 University Institute of Pharmacy, Pt. Ravishankar Sukla University, Raipur, C.G., IN

Abstract

The objective of the present study was to evaluate the synergistic antimicrobial and anti-tubercular activities of Opuntia dillenii aqueous methanolic extract. Antibacterial study was carried out by plate hole diffusion or agar well diffusion assay to determine the growth inhibition of bacteria. Antifungal activity was performed by the use of Saubouraud dextrose agar medium (SDA). The synergistic activity study was calculated by means of Cup plate method (Kirbauy bauer technique) using two wells in a plate. The aqueous methanolic plant extract of Opuntia dillenii (500 μg/ml) was used in combination with oxytetracycline (500 μg/ml). Anti-tubercular assay was performed using Micro plate Alamar Blue Assay (MABA) using the suspension of Mycobacterium tuberculosis H37Rv strain. The concentrations of plant extract were used are 1000 μg/ml, 500 μg/ml, 250 μg/ml, 125 μg/ml, 62.5 μg/ml. The medicinal plant appear to have a broad antimicrobial activity spectrum, they could be useful in antiseptic and disinfectant formulation as well as in anti-tubercular activity. Among the various micro organisms, the aqueous methanolic extract was more active against Micrococcus leuteum, Proteus mirabilis. In antifungal activity of the aqueous methanolic extract shows positive results for all fungus. The antitubercular activities were compared with standard drug Rifampicin. The aqueous methanolic extract was having more percentage inhibition when compared to other extracts.

Keywords

Opuntia dillenii, Mycobacterium tuberculosis, Micro Plate Alamar Blue Assay.

Full Text

     

G. Ganesh Kumar ¹, Raja Sekhar Mamillapalli ²

Affiliations
1 IIT, Bombay, IN
2 NICMAR, Hyderabad, IN

Abstract

In spite of substantial improvements in material strength and increased accuracy in analysis in Design and Analysis with the aid of digital computers, gradual detoriation of structures during prolonged usage cannot be completely ruled out. This has been proven by many recent accidents such as sudden Bridge Collapses and in-flight break downs of Aircrafts and shuttles, causing immense loss in lives and properties. Hence, the idea of equipping structures with sensors and actuators in an attempt to impart "smartness" has great potential in cost-effective predictive maintenance of structures, particularly for high performance components not easily accessible for manual inspections. There is a huge demand in finding out a new method of SHM in Civil Engineering Structures, which should be a feasible, low cost, light weight and appropriate method to predict the damage in advance to save the Lives and property. In this report, the importance of Smart materials, especially Piezoelectric materials in Various Applications in field of Engineering have been discussed. SHM principle and case studies related to SHM have been discussed. Electro-mechanical Impedance method and their applications in SHM of Civil Structures is explained along with a Case study of Utilization of Piezoelectric material in RC Structures in SHM applications have been discussed along with the discussion of results.

Full Text